Exon trapping

In an unidentified segment of DNA, a gene can
be recognized by the occurrence of coding segments
(exons). To find and isolate an exon, the
genomic fragment can be cloned in a vector that
consists of a strong promoter gene and a reporter
gene. An exon that is present is cut out by
means of the donor and acceptor splice signals
and expressed together with the genomic fragment
(exon trapping). cDNA is produced from
the mRNA and replicated by means of PCR. Finally,
the trapped exon can be sequenced